The HyStem(R)-HP Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture where slowly released growth factors are crucial in re-creating a stem cell niche. To affect specific cell performance growth factors or ECM proteins may be added to the HyStem-HP hydrogel. Unlike animal-derived ECM products this kit contains three fully chemically defined components which are nonimmunogenicHyStem(R)-HP - a thiol-modified hyaluronan (a major constituent of native ECM) carboxymethyl hyaluronic acid-thiopropanoyl hydrazide (CMHA-S CMHA-DTPH carboxymethyl hyaluronic acid-DTPH) with heparinthiopropanoyl hydrazide (HP-DTPH). HyStem-HP (CMHA-DTPH with HP-DTPH) contains 99.7 wt% CMHA-DTPH and 0.3 wt% HP-DTPH.Gelin-S(TM) - a thiol-modified gelatin (denatured collagen) carboxymethyl gelatin-thiopropanoyl hydrazide (GTN-DTPH carboxymethyl gelatin-DTPH)Extralink(R) - a thiol-reactive crosslinker polyethylene glycol diacrylate (MW